INTERNATIONAL JOURNAL OF TECHNOLOGY MANAGEMENT & HUMANITIES
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Title:New Validated Rp-HPLC Method For The Estimation of Afloqualone In Pharmaceutical Formulation
Abstract:A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Afloqualone in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column Column at ambient temperature. The mobile phase consisted of Acetonitrile:1% Acetic acid: Water(40:40:20 v/ v/v). The UV detection wavelength was 285nm and 20 µl sample was injected. The run time for Afloqualone is 6 min. The flow rate was found to be 1ml/min. The percentage recovery of the method was found to be 100.59%. The LOD and LOQ for Afloqualone was found to be 10µg/ml and 30µg/ml respectively.The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Afloqualone formulations, which could be the better choices compared to the reported methods of literature