New Validated Rp-HPLC Method For The Estimation of Afloqualone In Pharmaceutical Formulation

Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for
rapid assay of Afloqualone in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow
rate of 1ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column Column at
ambient temperature. The mobile phase consisted of Acetonitrile:1% Acetic acid: Water(40:40:20 v/ v/v).
The UV detection wavelength was 285nm and 20 μl sample was injected. The run time for Afloqualone is
6 min. The flow rate was found to be 1ml/min. The percentage recovery of the method was found to be
100.59%. The LOD and LOQ for Afloqualone was found to be 10μg/ml and 30μg/ml respectively.The
method was validated as per the ICH guidelines. The method was successfully applied for routine quality
control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the
routine quality control analysis of Afloqualone formulations, which could be the better choices compared
to the reported methods of literature